EPPENDORF 0030129504 Pcr Plate Lobind Semi Skirted Twin.tec 25X 250UL

( Brand: Eppendorf ), ( Manufacturer Part Number: 0030129504 ), ( Model: TWIN.TEC ), ( Intended Use/discipline: Biological Laboratory, Microbiological Laboratory ), ( Type: Pcr Plate 96 Lobind, Semi-skirted ), ( Included Items: 25x 250ul Colorless )

Review EPPENDORF Tw Inch Tec Pcr Plate Lobind Semi skirted 25x

The **Eppendorf 0030129504 Twin.tec PCR Plate (96-Well, Lobind Semi-Skirted, 25x250 L)** is a high-performance, ultra-low binding microplate designed to meet the stringent demands of modern molecular biology and PCR applications. Engineered with Lobind s patented surface technology, this plate minimizes non-specific binding of nucleic acids, proteins, and other biomolecules, ensuring reliable and reproducible results across a wide range of assays, including quantitative PCR (qPCR), real-time PCR, and DNA/RNA amplification. The semi-skirted design, featuring a low-profile rim, enhances ease of handling while preventing cross-contamination between wells, making it ideal for automated liquid handling systems and high-throughput workflows. Each well has a precise volume capacity of 25 L for the base and 250 L for the skirted portion, providing flexibility for both minimal reaction volumes and additional buffer or reagent storage when needed. Constructed from high-quality, transparent polypropylene, the plate offers excellent optical clarity, ensuring optimal fluorescence detection and consistent performance in real-time monitoring systems. Its flat-bottom design further ensures even distribution of reagents and precise thermal cycling, while the durable, non-absorbent material resists warping and maintains structural integrity under repeated thermal stress. Whether used in research laboratories, clinical diagnostics, or industrial bioprocessing, this plate combines precision engineering with superior material properties to deliver consistent, high-fidelity results in every experiment.

**Pros and Cons of buying a Eppendorf 0030129504 Twin.tec PCR Plate (96-Well, Lobind Semi-Skirted, 25x250 L)**

### **Pros**

1. **High-Quality Material and Compatibility**

- The plate is made by **Eppendorf**, a trusted brand known for precision and reliability in laboratory equipment. The **Lobind brand** (a division of Eppendorf) is particularly renowned for its low-binding, high-quality PCR plates, which minimize sample loss and contamination.

- It is designed for **PCR applications**, ensuring compatibility with most thermal cyclers and automated liquid handling systems.

2. **Low-Binding Surface**

- The **low-binding (LB) surface** reduces adsorption of nucleic acids, proteins, and other biomolecules to the plate walls, improving recovery and consistency in experiments. This is critical for sensitive assays like qPCR, where sample integrity is paramount.

- The **semi-skirted design** helps prevent cross-contamination between wells while still allowing easy pipetting.

3. **Optimal Volume Range**

- Standard PCR reactions (e.g., 25 L reactions scaled up to 250 L for master mixes).

- High-throughput screening where larger volumes may be needed.

- Compatibility with multi-channel pipettes for efficient sample preparation.

- The **deep well depth (250 L)** reduces evaporation and splashing during pipetting, which is beneficial for long incubations or automated workflows.

4. **Durability and Reusability**

- The plate is constructed from **high-quality polypropylene**, making it durable and resistant to chemicals (e.g., detergents, organic solvents) and repeated autoclaving (if sterilization is required).

- Unlike some disposable plates, this can be **washed and reused multiple times**, reducing long-term costs.

5. **Compatibility with Automation**

- The **flat-bottomed, semi-skirted design** is optimized for use with **automated liquid handlers, robotic pipetting systems, and PCR robots**, making it ideal for high-throughput laboratories.

- The **consistent well geometry** ensures reliable performance in robotic workflows where precision is critical.

6. **Sealing Options**

- While not included, the plate is designed to work with **Eppendorf s adhesive seals (e.g., Twin.tec PCR Plate Seals, 0030129505)** or other compatible sealing films, which help prevent contamination during thermal cycling.

- Sealing also reduces evaporation, improving the stability of reactions over extended cycles.

7. **Cost-Effectiveness for High-Throughput Work**

- Although the upfront cost may be higher than disposable plates, the **durability and low-binding properties** justify the investment for labs performing **repeated experiments or large-scale screening**.

- Reduces waste compared to single-use plates, aligning with sustainability goals.

---

### **Cons**

1. **Higher Initial Cost**

- The **premium quality** of Lobind/Eppendorf plates comes with a **higher price tag** compared to generic or disposable PCR plates. This may be a barrier for small labs or researchers on tight budgets.

- The cost per well is significantly higher than that of single-use plates, which might not be justified for one-time experiments.

2. **Maintenance and Cleaning Requirements**

- Unlike disposable plates, **reusable plates require thorough cleaning** between uses to prevent carryover of contaminants (e.g., DNA, proteins, or detergents). This adds time and effort to workflows.

- **Autoclaving or chemical cleaning** (e.g., with bleach or dedicated plate cleaners) is necessary to ensure sterility, which can degrade the plate over time if not done properly.

3. **Potential for Wear and Tear**

- With repeated use, the **plastic may become brittle or develop scratches**, which could affect sealing or pipetting accuracy. Over time, the plate may need replacement, adding to long-term costs.

- The **skirted design** can sometimes trap debris or liquid, requiring extra care during cleaning.

4. **Limited Compatibility with Some Seal Types**

- While the plate works with Eppendorf s adhesive seals, **third-party seals may not fit perfectly**, leading to leaks or inconsistent sealing. This requires careful selection of compatible sealing films.

- Some users report that **foil seals** (e.g., from other manufacturers) may not adhere as securely as Eppendorf s proprietary seals.

5. **Not Ideal for All Applications**

- For **low-volume or ultra-sensitive assays** (e.g., single-molecule PCR or extremely low-copy-number targets), even low-binding plates may introduce some background noise. In such cases, **specialized low-binding plates** (e.g., from companies like Axygen or Corning) might be preferable.

- The **250 L well size** may be excessive for some applications (e.g., ultra-low-volume PCR or multiplexing with limited reagents), making smaller plates (e.g., 100 L or 50 L) more practical.

6. **Storage and Handling Considerations**

- The plate must be **stored properly** (e.g., in a clean, dry environment) to avoid dust or chemical contamination, which can affect subsequent experiments.

- If not sealed or covered during storage, **dust or debris** can settle in the wells, requiring additional cleaning before use.

---

### **Conclusion**

The **Eppendorf 0030129504 Twin.tec PCR Plate (96-well, Lobind, semi-skirted, 25x250 L)** is an **excellent choice for laboratories prioritizing reliability, low-binding performance, and automation compatibility**. Its **durability, precision, and compatibility with high-throughput workflows** make it ideal for **research labs, core facilities, and industrial settings** where consistency and efficiency are critical. The **higher upfront cost and maintenance requirements** are justified for labs performing **repeated experiments or large-scale screening**, as they reduce waste and improve data quality.

However, for **budget-conscious users, one-time experiments, or ultra-sensitive applications**, cheaper disposable plates or smaller-volume plates may be more practical. The decision ultimately depends on **budget, workflow demands, and the sensitivity of the assays** being performed.

### **Recommendation**

- You work in a **high-throughput lab** with frequent PCR experiments.

- You require **low-binding performance** to minimize sample loss.

- You use **automated liquid handling systems** and need reliable, reusable plates.

- Your budget allows for **long-term cost savings** despite the higher initial investment.

- You are on a **tight budget** and need disposable plates for occasional use.

- Your experiments require **ultra-low-volume or specialized plates** (e.g., for single-molecule PCR).

- You lack the **time or resources** to properly clean and maintain reusable plates.

For most **serious PCR applications in well-equipped labs**, this Eppendorf plate is a **highly recommended investment** due to its superior performance and durability.

Details:

Software test results are available. Collectibles are rare, vintage, an no longer manufactured or supported by OEM. Meets OEM specifications. All hardware is tested verified working through manual or software tests.

Minor wear of labeling may be visible. AVRITEK will not guarantee to the life of batteries, light bulbs / lamps, or any other consumables that may be contained within an item. Please inquire for alternative shipping services other than what is offered in the listing. Software may not be loaded or configured.

May have broken or missing parts. No corrosion. Software not loaded or configured. Heavy use and age.

Repaired w/ OEM parts. Averred is R2 certified. No damage to latches or hinges, nor any missing parts, panels, bezels covers. Cosmetic blemishes include minor scratches and/or other surface imperfections from consistent use, but equipment is in very good condition.

Cosmetic blemishes are significant, such as multiple heavy dents, may require repair. Are prevalent. Cosmetic blemishes include minor scratches and/or other surface imperfections from consistent use, but equipment is in good condition overall. Damage to latches, hinges and keyboards, etc.

Loaded configured w/ legally licensed software for full operations. Secondary functions may not be tested or working. No hardware defects. This item has been evaluated for condition and function in accordance with R2v3 requirements.

Loaded configured w/ manufacturers legally licensed software for full operation. New, out of box, unused equipment removed from original packaging with no signs wear or alteration. Overall excellent cosmetic condition. Zero defects.

Damages to latches, hinges, keyboards, etc. No physical damage or defects. Minor dents and areas of discoloration from the removal tags. Minor cosmetic blemishes have no impact on the systems overall functionality or performance.

May be missing components or parts not essential to key functions. Fully cleaned and cosmetically repaired w/ no blemishes. No hardware or software defects. No missing or damaged components parts.

Verified removed from operation with no none defects in functionality. For materials recovery only. A subset of the primary functions device that an ordinary user expects to function are verified working through manual or software tests. May not include focus materials egg: battery that are working or tested.

EPPENDORF TWIN.TEC PCR Plate 96 LoBind Semi-Skirted w/ 25x 250uL. Alternative shipping services will incur the full charge for desired service. New, unused equipment in original packaging where factory seals have been broken. Used equipment that has been fully restored to like-new condition w/ warranty.

R2v3 Product Cosmetic. Cosmetic blemishes are consistent with wear, including multiple dents, discoloration, and light to heavy scratches. Part numbers and serial verified accurate.

All Functions Tested and verified working through software tests. Some parts, panels, bezels, or covers may be missing. May have light scratches or other minor blemishes from normal wear. AVRITEK AVR-SALES helps organizations to safely and profitably offload excess or off lease equipment provides business critical hardware life cycle services.

Moderate use and age. All functions tested verified working through software tests. Fully cleaned with all non-original labels removed. No missing parts.

part #: 0030129504 prices

• $52.00-$89.96

specifications plasticwarelabsuppy:

• brand: Eppendorf
• mpn: 0030129504
• model: Twin.tec
• intended use/discipline: Biological Laboratory, Microbiological Laboratory
• type: Pcr Plate 96 Lobind, Semi-skirted
• included items: 25x 250ul Colorless

general plasticwarelabsuppy:

• Medical, Lab Dental Supplies > Glassware Plasticware > Other Medical Lab Glassware
• condition: New

seller plasticwarelabsuppy:

• city: San Diego, California
• # reviews: 17399
• rating: 99.4%

returns plasticwarelabsuppy:

• time: 30 Days
• method: Money back
• policy: Returns Accepted
• paid by: Seller

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shipping plasticwarelabsuppy:

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offer plasticwarelabsuppy:

• best offers: True
• quantity: 1
• availability: In Stock
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• options: Ship-to-home
• started: February 13, 2026

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