INVITROGEN 12263-018 Purelink Plasmid Purification

( Brand: Invitrogen ), ( Manufacturer Part Number: 12263-018 )

Review INVITROGEN Purelink Plasmid Purification System

The Invitrogen PureLink 96 Plasmid Purification Kit (12263-018) is a powerful tool designed for the efficient and high-yield purification of plasmid DNA from E. coli bacterial cultures. This kit employs a unique combination of magnetic bead technology and proprietary resins to simplify and streamline your plasmid purification workflow.

The kit is specifically formulated to handle up to 96 samples at once, making it an ideal choice for large-scale or high-throughput plasmid purification projects. Each well in the 96-well plate is pre-loaded with magnetic beads coated with a highly specific and selective binding agent. This agent binds to the plasmid DNA, allowing for efficient separation from the host cell debris and unwanted nucleic acids.

The PureLink 96 Plasmid Purification Kit utilizes a simple, one-step protocol that involves adding the bacterial lysate to the wells, incubating for a short period, and then magnetically separating the beads to collect the purified plasmid DNA. The DNA is then eluted in a minimal volume of elution buffer, preserving its integrity and reducing the risk of contamination.

The kit is compatible with a wide range of plasmid types, including those containing insert sizes up to 10 kb, and it reliably removes salts, proteins, and other contaminants. The purified plasmid DNA is ready for downstream applications such as sequencing, ligation, and transformation.

The Invitrogen PureLink 96 Plasmid Purification Kit (12263-018) is known for its high yield, uniformity, and ease of use. It offers a reliable and scalable solution for plasmid DNA purification, helping to ensure the success of your experimental outcomes.

1. High purity: This kit uses magnetic beads that selectively bind to plasmid DNA, resulting in high purity plasmid DNA.

2. Scalability: It is designed for 96-well format, making it suitable for large-scale purification of plasmid DNA.

3. Convenience: The kit includes all the necessary reagents and buffers, making it a one-stop solution for plasmid DNA purification.

4. Fast and easy: The kit provides fast and simple plasmid DNA purification with minimal hands-on time.

1. Cost: The kit is relatively expensive compared to other plasmid DNA purification methods.

2. Requires specialized equipment: The kit requires a magnetic separator, which is not included in the kit, adding to the overall cost.

3. Limited capacity: The kit is designed for 96-well format, which may limit its capacity for purifying larger quantities of plasmid DNA.

The Invitrogen PureLink 96 Plasmid Purification Kit (12263-018) is a high-quality and convenient tool for purifying plasmid DNA in a large-scale format. Its high purity and fast and easy procedure make it an attractive option for researchers. However, its cost and requirement for specialized equipment may be a drawback for some users. Ultimately, the decision to purchase the kit should depend on the specific needs and budget of the researcher.

For researchers who require high-purity plasmid DNA and need to process a large number of samples in a short time, the Invitrogen PureLink 96 Plasmid Purification Kit (12263-018) is a recommended option. However, if budget and capacity constraints are a concern, other plasmid DNA purification methods may be more suitable.

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part #: 12263018 prices

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Ratings:

1. Excellent !! 10-24-17
2. EXTREMELY well packed THANK YOU!, quick ship, good stuff 10-23-17
3. Good tubing 10-25-17
4. very good. 10-21-17
5. fast and excellent product 10-23-17

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