
( Brand: Pierce ), ( Manufacturer Part Number: 13222 ), ( Part Type: Vial Box ), ( Country Of Origin: United States ), ( Intended Use/discipline: Reaction Vials )
The **Pierce 13222 Reacti-vial 3.0 mL Conic Reaction Vials** represent a sophisticated and versatile solution for researchers and laboratory professionals seeking precision, efficiency, and reliability in their biochemical and molecular biology experiments. Engineered with meticulous attention to detail, these conical reaction vials are part of Pierce s high-performance line of consumables, designed to meet the rigorous demands of modern laboratory workflows. Each vial in this 12-pack features a **3.0 mL capacity**, providing ample volume for a wide range of applications, including enzyme assays, protein purification, nucleic acid extraction, and small-scale biochemical reactions. The **conical shape** of the vials ensures optimal mixing and minimizes dead space, reducing the risk of sample loss and contamination while facilitating thorough resuspension of pellets or precipitates. Constructed from **high-quality, low-protein-binding polypropylene**, these vials are engineered to maintain sample integrity, preventing nonspecific adsorption of proteins, nucleic acids, or other biomolecules that could compromise experimental results. The material s durability and chemical resistance also make it compatible with a broad spectrum of buffers, detergents, and organic solvents, ensuring compatibility across diverse experimental protocols.
Beyond their functional attributes, the Reacti-vial 3.0 mL design incorporates practical features that enhance usability and workflow efficiency. The **screw-cap closure** is engineered with a secure, leak-proof seal to prevent evaporation and contamination during storage or transport, while the **flat-bottomed design** allows for stable placement in standard microcentrifuge tubes or reaction blocks, reducing the risk of tipping or spillage. The vials are also **sterile and DNase/RNase-free**, eliminating the need for additional purification steps and ensuring that experiments are not compromised by enzymatic degradation of nucleic acids. Additionally, the **clear polypropylene material** provides excellent visibility, enabling precise pipetting and monitoring of reactions without the need for additional lighting or magnification. Whether used in routine laboratory procedures or cutting-edge research, these vials are designed to integrate seamlessly into automated systems, manual pipetting workflows, or high-throughput screening applications, offering both flexibility and reliability.
For laboratories prioritizing consistency and reproducibility, the Pierce Reacti-vial 3.0 mL vials deliver a dependable performance that aligns with the highest industry standards. The batch-to-batch uniformity of the material ensures that experimental conditions remain constant, while the vials compatibility with common laboratory equipment such as thermocyclers, shakers, and centrifuges further streamlines experimental design. Whether conducting protein assays, PCR reactions, or cell culture manipulations, researchers can trust these vials to provide a stable, contamination-free environment for their samples. With their combination of **durability, chemical resistance, and ease of use**, the Pierce 13222 Reacti-vial 3.0 mL Conic Reaction Vials are an indispensable tool for any laboratory aiming to achieve accurate, efficient, and reproducible results.
**Pros and Cons of buying a Pierce 13222 Reacti-vial 3.0 mL Conic Reaction Vials (Box of 12)**
### **Pros:**1. **High-Quality Material:** The Reacti-vials are made from high-quality polypropylene, which is durable, chemically resistant, and suitable for a wide range of laboratory applications, including PCR, DNA/RNA extraction, and small-scale reactions.
2. **Conical Shape:** The conical design ensures efficient mixing and reduces evaporation, which is particularly useful for reactions requiring precise volume control or long incubation periods.
3. **Compatibility:** These vials are compatible with most standard laboratory equipment, including thermocyclers, shakers, and centrifuges, making them versatile for various workflows.
4. **Sterile and Leak-Proof:** The vials are typically sterile upon packaging and feature secure caps to prevent leaks, which is critical for maintaining sample integrity and preventing contamination.
5. **Cost-Effective for Small-Scale Work:** While not the cheapest option, the price per vial is reasonable for small-scale experiments, especially when purchased in bulk (e.g., a box of 12).
6. **Brand Reputation:** Pierce (now part of Thermo Fisher Scientific) is a trusted name in laboratory consumables, ensuring reliability and consistent performance.
7. **Easy to Handle:** The vials are lightweight and easy to manipulate, reducing user fatigue during repetitive tasks like pipetting or vortexing.
8. **Wide Application Range:** Beyond basic reactions, they can be used for enzyme assays, protein purification, and other biochemical procedures where precise volume handling is required.
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### **Cons:**1. **Limited Volume:** At only 3.0 mL, these vials are not suitable for large-scale reactions or experiments requiring higher volumes. Larger volumes may necessitate scaling up or using different containers.
2. **Single-Use Nature:** While durable, these vials are generally designed for single-use to prevent cross-contamination. This adds to disposal costs and environmental concerns if not recycled properly.
3. **Cost per Unit:** Compared to generic or lower-end brands, the cost per vial may be higher, which could be a drawback for labs on tight budgets or those performing high-throughput experiments.
4. **Cap Compatibility Issues:** Some users report that the caps may not fit perfectly with certain automated liquid handlers or custom setups, requiring additional adapters or adjustments.
5. **Not Ideal for High-Speed Centrifugation:** The conical shape and material may not be optimal for high-speed centrifugation, where heavier or more rigid vials might be preferred to avoid breakage or leakage.
6. **Limited Customization:** Unlike some other brands, these vials do not offer customizable features such as color-coding or special coatings, which could be useful for tracking or specific experimental needs.
7. **Potential for Evaporation:** While the conical shape helps, some volatile solvents or reagents may still evaporate over time, requiring careful monitoring or sealing with additional measures (e.g., parafilm).
8. **Supplier Dependence:** As a branded product, availability may depend on the supplier s inventory and lead times, which could disrupt workflows if stock runs low.
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### **Ending Conclusion:**The Pierce 13222 Reacti-vials are a reliable and high-quality choice for small-scale laboratory reactions, particularly in applications where mixing efficiency, sterility, and compatibility with standard equipment are priorities. Their conical design and durable construction make them well-suited for tasks like PCR, DNA/RNA work, and enzyme assays. However, their single-use nature, limited volume capacity, and higher cost compared to generic alternatives may not make them ideal for large-scale or high-throughput operations. Additionally, users must ensure compatibility with their specific equipment and consider disposal logistics.
### **Recommendation:**- **Buy if:** You need a dependable, sterile, and well-designed vial for small-scale reactions (e.g., PCR, DNA/RNA extraction, or enzyme assays) and prioritize quality and ease of use over cost savings. The Reacti-vials are particularly recommended for labs that frequently use thermocyclers or require precise volume control.
- **Consider alternatives if:** You require larger volumes, high-speed centrifugation, or cost-effective options for bulk use. In such cases, generic polypropylene vials or larger-capacity tubes (e.g., 5 mL or 15 mL) might be more appropriate. Additionally, if your workflow involves automated liquid handling, verify cap compatibility beforehand.
- **Budget Tip:** If cost is a concern, compare prices across suppliers (e.g., Thermo Fisher s website, Amazon, or lab supply distributors) and check for bulk discounts or promotions. Alternatively, evaluate whether generic vials meet your needs for less expensive options.
Overall, the Pierce Reacti-vials are a solid investment for labs that value performance and reliability in small-scale experiments.
Pierce Reacti-Vial 3.0mL Small conic flat bottom reaction Vials Box of 12 Cat# 13222, Comes from a Midwest research university.