
( Brand: Superdex ), ( Manufacturer Part Number: 17-1089-01 )
The Superdex 17-1089-01, a product of Cytiva (GE Healthcare), is a premium gel filtration column designed for superior separation and purification of proteins and other biomolecules. This particular column, modeled as a 200 3.2/300 GL, boasts a unique size-exclusion chromatography (SEC) technology that provides exceptional resolution and reproducibility in the analysis and purification of a wide range of biomolecules.
Crafted with a 1000 nominal pore size and a 7.8 x 30 cm column length, the Superdex 17-1089-01 is ideal for separating proteins within a molecular weight range of 10 kDa to 600 kDa. The column is pre-equipped with a 5 m nominal particle size, 300 pore size, and 0.65 cm (1/4") I.D. guard column, ensuring optimal protection and longevity of the analytical column.
The Superdex 17-1089-01 is compatible with a variety of buffers, including those containing up to 1 M salt and 50% organic solvents, making it a versatile tool for diverse biomolecule separation tasks. This column is optimized for use with the Akta purifier system, allowing for seamless automation and high-throughput purification of your biomolecules.
With its robust design, exceptional separation efficiency, and wide compatibility, the Superdex 17-1089-01 gel filtration column by Cytiva (GE Healthcare) is an indispensable tool for protein researchers and bioprocessing professionals, contributing to the success of your projects and advancing the field of biotechnology.
The Superdex 17-1089-01 Cytiva (GE Healthcare) 200 3.2/300 GL Column (Pharmacia) is a high-performance size exclusion chromatography (SEC) column used for the separation, analysis, and purification of biomolecules such as proteins, peptides, and nucleic acids. Here are some pros and cons of purchasing this column:
Pros:1. High-quality and reliable: The column is manufactured by Cytiva (GE Healthcare), a reputable company in the biotechnology industry known for producing high-quality chromatography products.
2. Wide separation range: The Superdex 17-1089 offers a wide molecular weight range (1.4 kDa - 660 kDa) for the separation of a broad range of biomolecules.
3. Excellent resolution: The column provides excellent resolution and reproducibility, which is crucial for accurate analysis and purification of biomolecules.
4. Easy to use: The column is designed for easy and quick use, making it suitable for both routine laboratory work and research applications.
5. Long lifespan: With proper care and maintenance, the column can be used for multiple separations, making it a cost-effective choice in the long run.
Cons:1. High price: The Superdex 17-1089 is a premium product and hence it comes with a relatively high price tag.
2. Requires specific buffers: The column requires specific buffers for operation, which may not be readily available or may be expensive.
3. Limited capacity: The column has a limited sample loading capacity, which may not be suitable for large-scale purification applications.
4. Not suitable for all sample types: The Superdex 17-1089 is designed for the separation of globular proteins and may not be ideal for the separation of other types of biomolecules such as membrane proteins or nucleic acids.
Conclusion:The Superdex 17-1089-01 Cytiva (GE Healthcare) 200 3.2/300 GL Column (Pharmacia) is a high-quality, reliable, and efficient tool for the separation, analysis, and purification of globular proteins. However, its high price and limited capacity may make it a less suitable choice for some applications. If you require a wide molecular weight range, excellent resolution, and reproducibility for your protein separation needs, then this column may be a worthwhile investment. If you are working on a tight budget or require a column for large-scale purification, you may want to consider alternative columns with lower prices and higher sample loading capacities.
Lot con5/5brownbxSG Used once Pharmacia/GE Healthcare Superdex 200 3.2/3 cat no 17-1089-01 column in metal cartridge.