
( Brand: Thermo Fisher Scientific ), ( Manufacturer Part Number: Q33120 ), ( Unit Type: Unit )
The Thermo Fisher Scientific Q33120 Invitrogen Quant-iT dsDNA Assay Kit - High Sensitivity is a cutting-edge, research-grade tool designed for precise and sensitive quantification of double-stranded DNA (dsDNA) in various samples. This assay kit, developed by the renowned Invitrogen brand and distributed by Thermo Fisher Scientific, boasts a high sensitivity that allows for detection of dsDNA as low as 10ng per sample, making it an ideal choice for low-input or precious samples.
The Quant-iT dsDNA Assay Kit employs a unique, fluorometric methodology that utilizes a dye specifically designed to bind to dsDNA. This results in a measurable fluorescence intensity that is directly proportional to the dsDNA concentration in the sample. The assay protocol is straightforward, and it can be easily performed in a microplate reader, ensuring rapid and accurate results.
One of the key advantages of this kit is its broad dynamic range, which spans from 10 ng/mL to 1000 ng/mL. This makes it suitable for a wide variety of applications, from low-abundance DNA samples in molecular biology research to high-concentration DNA samples in forensic or clinical settings. Furthermore, the assay is highly reproducible, ensuring consistent results across multiple experiments and sample types.
The Quant-iT dsDNA Assay Kit - High Sensitivity comes complete with all necessary reagents and buffers, including the Quant-iT dsDNA Assay Buffer, Quant-iT dsDNA Assay Reagent, and Quant-iT dsDNA Standard. The standard provides a series of dsDNA concentrations for generating a standard curve, allowing for accurate quantification of unknown samples.
In summary, the Thermo Fisher Scientific Q33120 Invitrogen Quant-iT dsDNA Assay Kit - High Sensitivity is an essential tool for researchers and scientists who require precise and sensitive quantification of dsDNA. Its high sensitivity, broad dynamic range, and straightforward protocol make it a versatile and reliable choice for a wide array of applications.
The Thermo Fisher Scientific Q33120 Invitrogen Quant-iT dsDNA Assay Kit High Sensitivity is a tool for quantifying double-stranded DNA (dsDNA) in a solution. It's known for its high sensitivity, making it suitable for a wide range of applications. Here are some pros and cons to help you make an informed decision:
Pros:1. High Sensitivity: The kit is designed to quantify dsDNA with high sensitivity, making it ideal for low-dsDNA concentration samples.
2. Broad Linearity Range: The assay can accurately measure dsDNA concentrations from 5 ng/ L to 1 g/ L, providing a wide range of usability.
3. Easy to Use: The kit includes a simple, one-step protocol that requires minimal hands-on time, making it user-friendly.
4. Reliable Results: The assay is based on a fluorescence-based method, which ensures accurate and reproducible results.
Cons:1. Cost: The kit can be relatively expensive compared to other DNA quantification methods, which might be a deterrent for some users.
2. Requires Specific Equipment: The kit requires a fluorescence plate reader for measurement, which might not be available in all labs.
3. Limited Sample Types: The assay is designed for quantifying dsDNA, so it may not be suitable for RNA or protein quantification.
In conclusion, the Thermo Fisher Scientific Q33120 Invitrogen Quant-iT dsDNA Assay Kit High Sensitivity is a reliable and sensitive tool for quantifying dsDNA. However, its high cost and the need for specific equipment might be a concern for some users. If you frequently work with low-dsDNA concentration samples and have the necessary equipment, this kit could be a valuable addition to your lab. If cost is a concern or if you work with a variety of sample types, you might want to consider other DNA quantification methods or kits that offer more versatility at a lower cost.
Used, see photos. About 1/2 of 250ml bottle Quant-iT dsDNA HS buffer remaining. This DNA assay kit is highly selective for double-stranded over RNA, and in the ranges of 0.2 100 nag fluorescence signal linear. Provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards.
Thermo Invitrogen Q33120 Quant-iT dsDNA Assay Kit High Sensitivity.