
( Brand: Waters ), ( Manufacturer Part Number: 186001472 ), ( Part Type: Box )
The 186001472 RP 250mm 4.6 x 3.5um Waters HPLC Xterra RP18 Column is a high-performance liquid chromatography (HPLC) column designed for separating and analyzing complex mixtures of compounds. This particular column is manufactured by Waters, a renowned company known for its high-quality chromatography products.
The Xterra RP18 column is made of a material called C18, which is a type of silica-based stationary phase. The 18 in the product name refers to this type of material. The RP stands for reversed-phase, indicating that this column is optimized for separating non-polar compounds.
The column has a length of 250mm and an internal diameter of 4.6mm. The 250mm length provides a sufficient separation distance, while the 4.6mm internal diameter allows for a higher sample load, making it suitable for both research and industrial applications.
The particle size of the stationary phase is 3.5um, which is relatively small. Smaller particle sizes result in better resolution and increased efficiency, as they provide more surface area for interactions between the stationary phase and the analytes.
The 3.5um particle size of this column also makes it suitable for use with ultra-performance liquid chromatography (UPLC), a faster and more efficient variant of HPLC, due to its ability to achieve higher pressures.
The Xterra RP18 column is a versatile tool for a wide range of applications, including peptide mapping, small molecule analysis, and metabolomics. It is compatible with various mobile phases and can be used in both isocratic and gradient elution modes.
In summary, the 186001472 RP 250mm 4.6 x 3.5um Waters HPLC Xterra RP18 Column is a high-quality, versatile, and efficient tool for HPLC separations. Its small particle size and compatibility with UPLC make it a powerful instrument for a wide range of analytical applications.
Pros of buying a 186001472 rp 250mm 4.6 3.5um Waters HPLC Xterra RP18 column:1. High-quality material: The column is made of high-quality materials, ensuring its durability and reliability.
2. Excellent separation efficiency: The 3.5um particle size of the column provides excellent separation efficiency, allowing for the separation of complex mixtures.
3. Wide pH range: The column can handle pH ranges from 1 to 14, making it suitable for a wide range of applications.
4. Reproducible results: The column provides consistent and reproducible results, ensuring the accuracy of your analyses.
Cons of buying a 186001472 rp 250mm 4.6 3.5um Waters HPLC Xterra RP18 column:1. High cost: The column is expensive compared to other HPLC columns on the market.
2. Limited capacity: The column has a limited capacity, making it unsuitable for large-scale analysis.
3. Requires specialized knowledge: The use of the column requires specialized knowledge and training, which may not be available to all users.
4. Potential for column degradation: The column can degrade over time, requiring regular maintenance and replacement.
Conclusion:The 186001472 rp 250mm 4.6 3.5um Waters HPLC Xterra RP18 column is a high-quality product that provides excellent separation efficiency and reproducible results. However, its high cost and limited capacity may make it unsuitable for some users. If you have the budget and the necessary expertise, this column can be a valuable addition to your HPLC system.
Recommendation:If you are looking for a high-quality HPLC column for complex mixture separations, the 186001472 rp 250mm 4.6 3.5um Waters HPLC Xterra RP18 column is a great option. However, if cost is a concern, you may want to consider other options that offer similar separation efficiency at a lower price. It is always recommended to do thorough research and compare different columns based on your specific application requirements before making a purchase.
6 x 250mm in sealed box. New Waters xterra rp hplc column RP18 3.