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  3. BIO RAD 731-1550

BIO-RAD 731-1550 Poly Chromatography Columns Caps Reservoir 10ML

731-1550 Bio-rad 44 Poly-prep Chromatography Columns With Caps 10ml Reservoir

( Brand: Bio-rad ), ( Manufacturer Part Number: 731-1550 ), ( Part Type: Cap ), ( Intended Use/discipline: Biological Laboratory, Dental Laboratory, Medical Laboratory, Microbiological Laboratory, Physical Laboratory ), ( Country Of Origin: United States ), ( Unit Quantity: 44 ), ( Unit Type: Unit ), ( Material: Polypropylene, Polyethylene ), ( Volume: 10ml, 0-2ml ), ( Additional Search Terms: Surplus Laboratory Bench Supplies Consumables )

Review BIO RAD Poly prep Chromatography Columns Caps 10ml Reservoir

The **Bio-Rad Poly-Prep Chromatography Columns with 10 mL Reservoir and 731-1550 Cap** are essential components for efficient and reliable biochemical separations, designed to streamline a wide range of laboratory procedures involving gel filtration, ion exchange, affinity chromatography, and other column-based purification techniques. Engineered with precision and durability, these columns feature a robust polypropylene construction that ensures stability and resistance to chemical corrosion, making them suitable for use with a variety of buffers, solvents, and detergents commonly employed in molecular biology, biochemistry, and protein purification workflows. The **10 mL reservoir** provides ample volume for sample loading and buffer exchange, accommodating both small-scale and intermediate-scale applications without compromising resolution or yield. The **731-1550 cap** is equipped with a secure, leak-proof seal that prevents contamination and ensures consistent flow rates during elution, while its ergonomic design allows for easy attachment and detachment, reducing the risk of spills or leaks during handling. This cap also includes a built-in filter to retain small particles, further enhancing the purity of the collected fractions. Whether used in conjunction with Bio-Rad s own chromatography media or third-party resins, these columns deliver consistent performance, reproducibility, and ease of use, making them a trusted choice for researchers seeking high-quality separations in academic, industrial, and clinical settings. Their compatibility with standard laboratory equipment, such as peristaltic pumps and fraction collectors, ensures seamless integration into automated or semi-automated workflows, saving valuable time and minimizing manual intervention. Overall, the **Bio-Rad Poly-Prep Chromatography Columns with 10 mL reservoir and 731-1550 cap** represent a reliable, versatile, and cost-effective solution for laboratories demanding precision and efficiency in biochemical separations.

**Pros and Cons of Buying Bio-Rad 731-1550 Poly-Prep Chromatography Columns with a 10 mL Reservoir**

### **Pros**

1. **High Efficiency and Separation Performance** Poly-Prep chromatography columns are designed for efficient separation of proteins, nucleic acids, and other biomolecules. The glass wool and resin bed provide excellent flow characteristics and high resolution, making them ideal for preparative and analytical applications.

2. **Versatility in Applications** These columns are compatible with a wide range of chromatography techniques, including ion exchange, size exclusion, and affinity chromatography. They can be used for desalting, buffer exchange, and purification of various biomolecules.

3. **Durability and Reusability** The glass construction of the columns is sturdy and resistant to chemical degradation, allowing for repeated use with proper cleaning and regeneration. This makes them cost-effective in the long run, especially for laboratories with frequent chromatography needs.

4. **Compatibility with Common Resins** The columns work well with standard chromatography resins such as Sephadex, Sepharose, and DEAE-cellulose, which are widely available and well-characterized. This ensures flexibility in choosing the right resin for specific applications.

5. **Easy to Use and Maintain** The design is simple, with a 10 mL reservoir that allows for easy loading and elution of samples. The glass wool plug prevents clogging while maintaining flow rates, reducing the need for complex maintenance procedures.

6. **Cost-Effective for Small- to Medium-Scale Purifications** While the initial cost may be higher than disposable alternatives, the reusability and durability make them economical for laboratories that perform frequent purifications without needing large-scale automation.

7. **Compatibility with Standard Laboratory Equipment** These columns fit standard chromatography stands and can be used with peristaltic pumps or gravity flow, making them adaptable to various laboratory setups.

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### **Cons**

1. **Limited to Small-Scale Applications** The 10 mL reservoir capacity restricts their use to small-scale purifications. For larger volumes or high-throughput applications, larger columns or alternative systems (e.g., gravity flow columns with bigger reservoirs) may be required.

2. **Manual Handling Required** Unlike automated systems, these columns require manual assembly, sample loading, and elution, which can introduce variability and increase the risk of contamination if not handled carefully.

3. **Potential for Leakage or Clogging** If not assembled properly or if the glass wool becomes damaged, leakage or uneven flow rates can occur. Additionally, improper handling of resins can lead to clogging, reducing efficiency.

4. **Time-Consuming for Multiple Runs** Each run requires disassembly, cleaning, and reassembly of the column, which can be time-consuming if multiple purifications are needed in a short period. This may not be ideal for high-throughput laboratories.

5. **Limited to Gravity or Low-Pressure Flow** These columns are not designed for high-pressure chromatography systems. If higher flow rates or pressure are required, alternative systems such as FPLC or AKTA purification systems would be more appropriate.

6. **Maintenance and Cleaning Requirements** While durable, the columns require thorough cleaning and regeneration between uses to prevent cross-contamination and ensure optimal performance. This adds an extra step to the workflow.

7. **Cost of Replacement Parts** If components such as the glass wool or column seals wear out, replacements may be needed, adding to the long-term cost.

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### **Conclusion**

The **Bio-Rad 731-1550 Poly-Prep Chromatography Columns with a 10 mL reservoir** are an excellent choice for laboratories requiring **small- to medium-scale, high-efficiency biomolecule purification** with **reusability and versatility**. Their durability, compatibility with common resins, and ease of use make them a practical option for research settings where automation is not necessary. However, they are **not ideal for large-scale or high-throughput applications**, nor are they suitable for high-pressure chromatography. The trade-off between cost savings from reusability and the time investment in manual handling must be considered based on the specific needs of the laboratory.

### **Recommendation**

- **Buy if:** You need a **reliable, cost-effective, and reusable** chromatography system for **small-scale purifications** (e.g., protein desalting, nucleic acid separation, or preparative chromatography in a research lab). The columns are particularly useful for applications where **gravity flow or low-pressure systems** are sufficient.

- **Avoid if:** You require **large-volume purifications**, **high-throughput automation**, or **high-pressure chromatography**. In such cases, consider **larger gravity flow columns (e.g., 50 mL or 100 mL reservoirs)** or **automated systems (e.g., FPLC, AKTA, or Bio-Rad s BioLogic systems)**.

- **Alternative Considerations:**

- For **larger volumes**, Bio-Rad s **Poly-Prep Chromatography Columns with bigger reservoirs (e.g., 50 mL or 100 mL)** may be more suitable.

- For **automated workflows**, invest in **FPLC or AKTA systems**, which offer higher precision and scalability.

- For **disposable or single-use applications**, consider **pre-packed disposable columns** if contamination risk is a concern.

Ultimately, these columns are a **strong choice for academic and small-scale research laboratories** where **manual, reusable chromatography** is preferred over automated or disposable systems.

Details:

BIOTECH BARGAINS item for sale. 2 ml bed volume and 10 reservoir. Lure end fitting with snap-off tip. New, open partial pack of 44 columns with caps and tip closures.

Lab Bench Supplies. and Noah compatible. Porous 30 m polyethylene bed support retains fine particles.

part #: 7311550 prices

  • $54.67-$300.00

specifications plasticwarelabsuppy:

  • brand: Bio-rad
  • mpn: 731-1550
  • part type: Cap
  • intended use/discipline: Biological Laboratory, Dental Laboratory, Medical Laboratory, Microbiological Laboratory, Physical Laboratory
  • country of origin: United States
  • unit quantity: 44
  • unit type: Unit
  • material: Polypropylene, Polyethylene
  • volume: 10ml, 0-2ml
  • additional search terms: Surplus Laboratory Bench Supplies Consumables

general plasticwarelabsuppy:

  • Medical, Lab Dental Supplies > Glassware Plasticware > Other Medical Lab Glassware
  • condition: New Open box

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  • started: April 9, 2026
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part type: cap, intended use/discipline: biological laboratory, dental laboratory, medical laboratory, microbiological laboratory, physical laboratory, country of origin: united states, unit quantity: 44, unit type: unit, volume: 10ml, 0-2ml, additional search terms: surplus laboratory bench supplies consumables,
category: business & industrial > medical, lab dental supplies > glassware plasticware > medical lab glassware, material: polypropylene, polyethylene, sku: 13646945559390723,
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